Duilio Cascio, Ph.D.

I am Associate Director/Manager of the UCLA-DOE Macromolecular Crystallization and X-ray Crystallography Facilities at UCLA. My research interests involve the determination of the structure-functional relationships in proteins using crystallography approaches. I have been working in this area for more than 25 years. I have been collaborating with Dr. Kurtz’s UCLA team on the crystallography studies of the major erythrocyte membrane protein anion exchanger 1 (AE1). These efforts by the team have led to the generation of human AE1 crystals that we are now studying.  My experience in protein crystallography, coupled with the important transporters we are characterizing structurally, makes me excited about my long-term involvement with the outstanding scientists who are part of the UCLA team.

 

 

Recent and Selected Publications

Faham, S., Watanabe, A., Besserer, G.M., Cascio, D., Specht, A., Hirayama, B.A., Wright, E.M., Abramson, J. (2008) The crystal structure of a sodium galactose transporter reveals mechanistic insights into Na+/sugar symport. Science 321:810-814. PMID: 18599740.

Ujwal, R., Cascio, D., Colletier, J.P., Faham, S., Zhang, J., Toro, L., Ping, P., Abramson, J. (2008) The crystal structure of mouse VDAC1 at 2.3Å resolution reveals mechanistic insights into metabolite gating. Proc. Nat. Acad. Sci. USA 105: 17742-17747. PMID: 18988731. PMCID: PMC2584669.

Wiltzius, J.J., Landau, M., Nelson, R., Sawaya, M.R., Apostol, M.I., Goldschmidt, L., Soriaga, A.B., Cascio, D., Rajashankar, K., Eisenberg, D. (2009) Molecular mechanisms for protein-encoded inheritance. Nat. Struct. Mol. Biol. 16: 973-978. PMID: 19684598.

Ujwal, R., Cascio, D., Chaptal, V., Ping, P., Abramson, J. (2009) Crystal packing analysis of murine VDAC1 crystals in a lipidic environment reveals novel insights on oligomerization and orientation. Channels 3: 167-170. PMID: 19574737.

Miallau, L., Faller, M., Chiang, J., Arbing, M., Guo, F., Cascio, D., Eisenberg, D.J. (2009) Structure and proposed activity of a member of the VapBC family of toxin-antitoxin systems. VapBC-5 from Mycobacterium tuberculosis. J. Biol. Chem. 284: 276-283. PMID: 18952600. PMCID: PMC2610494.

Senturia, R., Faller, M., Yin, S., Loo, J.A., Cascio, D., Sawaya, M.R., Hwang, D., Clubb, R.T., Guo, F. (2010) Structure of the dimerization domain of DiGeorge critical region 8. Protein Sci. 19:1354-1365. PMID: 20506313.

Laganowsky, A., Benesch, L.P.J., Landau, M., Ding, L., Sawaya, M., Cascio, D., Huang, Q., Robinson, C.V., Horwitz, J., Eisenberg, D.S. (2010) Crystal structures of truncated alphaA and alphaB crystallins reveal structural mechanisms of polydispersity important for eye lens function. Protein Sci. 19: 1031-1043. PMID: 20440841. PMCID: PMC2868245.

Balendiran, G.K., Sawaya, M.R., Schwarz, F.P., Ponniah, G., Cuckovich, R., Verma, M., Cascio, D. (2010) The role of Cys298 in aldose reductase function. J. Biol. Chem. 2010 Nov 17. [Epub ahead of print]. PMID: 21084309.

 

mVDAC1 antiparallel dimer

mVDAC1 antiparallel dimer