Natalia Abuladze, Ph.D.

I have been involved over the last decade in the cloning and expression of SLC4 transporters. Our experiments led to the discovery of the human membrane transporter NBCe1-B that plays an important role in most human organs. I have played a key role in the discovery of the first electroneutral sodium bicarbonate cotransporter NBCn1, and the second electrogenic sodium bicarbonate cotransporter NBCe2. I have participated in the large-scale expression of human SLC4 transporters in insect and yeast expression systems in order to perform their high-resolution structural characterization. I have been involved in the crystal trials that have led to the first crystals of bacterial SbtA and mammalian AE1 and NBCe1-A.  I have participated in the purification of AE1, NBCe1-A and NBCn1 used by our team members for structural characterization using electron microscopy and 3D reconstruction approaches.

 

Recent and Selected Publications

Abuladze, N., Lee, I., Newman, D., Hwang, J., Boorer, K., Pushkin, A., Kurtz, I. (1998) Molecular cloning, chromosomal localization, tissue distribution, and functional expression of the human pancreatic sodium bicarbonate cotransporter. J. Biol. Chem. 273: 17689-17695. PMID: 9651366.

Pushkin, A., Abuladze, N., Lee, I., Newman, D., Hwang, J., and Kurtz, I. (1999) Cloning, tissue distribution, genomic organization, and functional expression of NBC3, a new member of the sodium bicarbonate cotransporter family. J. Biol. Chem. 247: 16569-16575. PMID: 10347222.

Abuladze, N., Song, M., Pushkin, A., Newman, D., Lee, I., Nicholas, S., and Kurtz, I. (2000) Structural organization of the human NBC1 gene: kNBC1 is transcribed from an alternative promoter in intron 3. Gene 251: 109-122. PMID: 10876088.

Pushkin, A., Abuladze, N., Newman, D., Lee, I., Xu, G., Kurtz, I. (2000) Cloning, characterization, and chromosomal assignment of NBC4, a new member of the sodium bicarbonate cotransporter family. Biochim. Biophys. Acta 1493: 215–218. PMID: 10978526.

Abuladze, N., Pushkin, A., Tatishchev, S., Newman, D., Sassani, P., Kurtz, I. (2004) Expression and localization of rat NBC4 in liver and renal uroepithelium. Am. J. Physiol. 559: 55-65. PMID: 15151908.