Rustam Azimov, Ph.D

I have beeen involved in the functional characterization of SLC4 transporters using measurements of intracellular pH  in the mammalian HEK expression system.  Proximal renal tubular acidosis is a disease that severely affects the ability of the proximal tubule to absorb bicarbonate. The electrogenic sodium bicarbonate cotransporter NBCe1-A plays an important role. We have characterized the importance of TM1 in NBCe1-A and have studied the functional abnormalities in transporters expressing natural NBCe1-A mutations.  These studies are aimed at defining the transport site.  We use various methodologies including cysteine scanning with MTS reagents to uncover the molecular transport characteristics of the cotransporter.

 

Recent and Selected Publications

Kao, L., Sassani, P., Azimov, R., Pushkin, A., Abuladze, N., Peti-Peterdi, J., Liu, W., Newman, D., Kurtz, I.  (2008) Oligomeric structure and minimal functional unit of the electrogenic sodium bicarbonate cotransporter  NBCe1-A. J. Biol. Chem. 283: 26782-26794. PMID18658147. PMCID: PMC2546552.

Zhu Q, Azimov R, Kao L, Newman D, Liu W, Abuladze N, Pushkin A, Kurtz I.(2009) NBCe1-A Transmembrane Segment 1 Lines the Ion Translocation Pathway.J Biol Chem. 2009 Mar 27;284(13):8918-29. Epub 2009 Jan 21. PMID: 19158093

Zhu, Q., Kao, L., Azimov, R., Newman, D., Liu, W., Pushkin, A., Abuladze, N., Kurtz, I. (2010) Topological location and structural importance of the NBCe1-A residues mutated in proximal renal tubular acidosis. J. Biol. Chem. 285:13416–13426. PMID: 20197274. PMCID: PMC2859501.

Zhu Q, Kao L, Azimov R, Abuladze N, Newman D, Pushkin A, Liu W, Chang C, Kurtz I.J Biol Chem. (2010) Structural and functional characterization of the C-terminal transmembrane region of NBCe1-A. Nov 26;285(48):37178-87. Epub 2010 Sep 13. PMID: 20837482